Journal: eLife

Article Title: N-cadherin-regulated FGFR ubiquitination and degradation control mammalian neocortical projection neuron migration

doi: 10.7554/eLife.47673

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-HA.11 clone 16B12 (Mouse monoclonal) , Eurogenetic , Cat# MMS-101R-500, RRID: AB_10063630 , WB (1:8000) IF (1:100) IP (1:400).

Techniques: FLAG-tag, Recombinant, Sequencing, Protease Inhibitor, Plasmid Preparation, DNA Purification, Gel Extraction, Software, Staining, In Vitro, Transfection

Journal: Frontiers in Immunology

Article Title: Adenovirus vector-mediated single chain variable fragments target the nucleocapsid protein of porcine epidemic diarrhea virus and protect against viral infection in piglets

doi: 10.3389/fimmu.2023.1058327

Figure Lengend Snippet: Jejunal expression of four scFvs. After rAdV-ZW, rAdV-wild-type or PBS oral administration, jejunums were collected on days 2 ( Aa , Ba ), 10 ( Ab , Bb ), 14 ( Ac , Bc ), and 18 ( Ad , Bd ). (A) Western blotting of scFv expression in jejunum tissue showing several bands at approximately 30-35 kDa. Lanes 1–3, jejunum tissue lysate from piglets administrated rAdV-ZW (lane 1), rAdV-wild-type (lane 2), and PBS (lane 3). (B) Immunofluorescence analysis showing scFv expression in jejunum tissue. Jejunum paraffin sections were incubated with a primary mouse monoclonal anti-HA antibody to detect HA-tagged scFvs, and then stained with DAPI to observe nuclei. Red fluorescence represents scFvs and blue represents nuclei. Scale bar = 100 μm. (C) Immunohistochemical jejunum tissue staining to analyze scFv expression. Brown staining indicates scFvs and blue represents hematoxylin-stained nuclei. Scale bar = 100 μm.

Article Snippet: After blocking in 5% skim milk, membranes were incubated with a primary mouse monoclonal anti-HA antibody (CMCTAG, Milwaukee, WI, USA) overnight at 4°C, and then incubated with a horseradish peroxidase (HRP)-conjugated Affinipure goat anti-mouse IgG (H + L) antibody (Jackson ImmunoResearch, West Grove, PA, USA) at 37°C for 1 h. Protein bands were revealed using Immobilon Western Chemiluminescent HRP Substrate (Merck-Novagen, Darmstadt, Germany).

Techniques: Expressing, Western Blot, Immunofluorescence, Incubation, Staining, Fluorescence, Immunohistochemical staining